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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (2,270)
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DNA Polymerases (1,098)
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dNTPs (263)

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Keyword Search:
91 – 105 of 4,526 results
91

Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type Hot Start DNA Polymerase
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
92

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
93
Promotions Available

Applied Biosystems™ TaqPath™ ProAmp™ Multiplex Master Mix
GSA_VA

TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C in the dark.
For Use With (Application) Genotyping,Pharmacogenomics
PCR Method qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Product Type qPCR Master Mix
Passive Reference Dye Mustang Purple
For Use With (Equipment) QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7 Real-Time PCR System ViiA 7 Dx, 7500 Fast, StepOne or StepOnePlus
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Very High
Target Specificity cDNA or gDNA only
Product Line TaqPath
94
Promotions Available

Applied Biosystems™ SteriSEQ™ Rapid Sterility Testing Kit

The SteriSEQ Rapid Sterility Testing Kit is a TaqMan-based quantitative PCR (qPCR) kit for the detection of bacteria and fungi in complex bioproduction samples.

Content And Storage • SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light
• qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light
• SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C
• SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature
Form Liquid
Label or Dye FAM + VIC,ABY,Alexa Fluor 647
For Use With (Application) Real Time PCR (qPCR)
PCR Method qPCR
Product Line SteriSEQ
Detection Method qPCR
95

Thermo Scientific™ Biotin-11-dUTP Solution (1 mM)
GSA_VA

Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.

96

Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
GREENER_CHOICE

The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.

ENCOMPASS_PREFERRED
Shipping Condition Frozen, Dry Ice
GC-Rich PCR Performance High
Form Liquid
Isolation Technology Direct Cell Lysis
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Target cDNA
Format Kit
Product Type Direct Cell Lysis to RT-qPCR Kit
Sample Type Hepatocytes, HepaRG, HepG2
Reaction Speed 10 min.
Label or Dye ROX
Purification Target RNA
Starting Material Up to 100,000 Cultured Cells
97
Promotions Available

Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kit with PrepSEQ™ Express Nucleic Acid Extraction Kit

The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.

98
Promotions Available

Invitrogen™ Platinum™ II Green PCR Buffer (5X)

Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR

ENCOMPASS_PREFERRED
100

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
GSA_VA

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
101
Promotions Available

Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)

GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.

ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Form Liquid
Concentration 10 mM
For Use With (Application) Standard PCR
Product Line GeneAmp
102

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

ENCOMPASS_PREFERRED
103

Thermo Scientific™ dCTP Solution (100 mM)
GSA_VA

Thermo Scientific™ dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

104

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Format Master Mix
Size (Final Product) Up to 20 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
105

Applied Biosystems™ TaqMan™ QSY and QSY2 Probes
GREENER_CHOICE

Applied Biosystems TaqMan QSY and QSY2 probes are dual-labeled probes used for real-time PCR. TaqMan QSY and QSY2 Probes are designed for optimal sensitivity and performance in multiplexed qPCR experiments for detection of up to 6 targets.

ENCOMPASS_PREFERRED
Shipping Condition Room Temperature
Purification Method HPLC
Format Tube
Internal Probe Modification None
Form Liquid or Dried Down
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe