PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

91 – 105 of 3,710 results

Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

Pricing & Availability
Specifications

Polymerase	DNA Polymerase I
Content And Storage	DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂ , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
Hot Start	No

Invitrogen™ MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

Pricing & Availability

Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX

VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.

Pricing & Availability
Specifications

Polymerase	AmpliTaq™ Fast DNA Polymerase
Activation Time	5 min.
Final Product Type	Mastermix
Form	Liquid
Reaction Speed	Fast
Label or Dye	ROX
Hot Start	Built-in Hot Start
Concentration	2X
Starting Material	DNA/RNA
Compatibility	TaqMan™
For Use With (Application)	Animal Health
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe

Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

Pricing & Availability

Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Pricing & Availability

Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RT-PCR Assay Kit

Detect RNA from multiple respiratory viruses simultaneously with the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit, a highly accurate, real-time PCR multiplex assay that allows you to expand your respiratory sample analysis menu while maintaining low operational costs.

Pricing & Availability

Invitrogen™ Second Strand cDNA Synthesis Kit

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Pricing & Availability
Specifications

Reaction Format	Separate Components
Final Product Type	Double Stranded cDNA
Product Type	cDNA Synthesis Kit
For Use With (Application)	Cloning

Promotions Available

Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR

Invitrogen™ DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Polymerase	DNA Polymerase I
Description	DNA Polymerase
Hot Start	No
Exonuclease Activity	3' - 5', 5' - 3'

100

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Pricing & Availability
Specifications

Fidelity (vs. Taq)	1X
Format	Stand-alone enzyme
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Overhang	3'-A
Concentration	5 U/μL
Starting Material	DNA
Exonuclease Activity	5' - 3'

101

Invitrogen™ PureLink™ PCR Micro Kit

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Separation Range	100 bp to 12 kb
Content And Storage	Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Starting Material Volume	125 μL
Isolation Technology	Silica Spin Column
Elution Volume Max	20 μL
For Use With (Application)	Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Target	DNA from Enzymatic Reactions (e.g. PCR)
Format	Spin Column
Product Type	PCR Micro Kit
Run Time	10 min.
Elution Volume Min	5 μL
Product Line	PureLink

102

Promotions Available

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	Quantitative PCR SuperMix-UDG
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Platinum
Detection Method	Primer-probe

103

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	PCR Amplified cDNA
PCR Method	1-step RT-PCR
Detection Method	Gel Electrophoresis
Polymerase	Platinum Taq High Fidelity
Format	One-Step RT-PCR System
Size (Final Product)	Up to 10 kb
Reaction Format	Premixed Components
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Hot Start	Built-In Hot Start
Optimal Reaction Temperature	50°C
Technique	1-Step RT-PCR
Starting Material	RNA

104

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Purity or Quality Grade	LD (Low DNA)
Reverse Transcriptase	M-MLV
Purification Time	5 min.
Concentration	20X
For Use With (Application)	RT-PCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Cells
Reaction Speed	Standard
Label or Dye	SYBR Green
Hot Start	Built-In Hot Start
Product Line	Cells-to-CT, SYBR

105

Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase

Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Tsp Genotyping Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Size (Final Product)	500 bp or less
Reaction Format	Separate Components
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	Blunt
For Use With (Application)	Hot-start PCR

Welcome to fishersci.com

PCR Supplies

Filtered Search Results

Narrow Results

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit